Apimedica: Royal Jelly Free of Estrogens, Does Not Promote Growth of Breast Cancer - 蜂王浆不含有雌性激素，并且不会促进小鼠体内MCF-7 乳腺癌细胞的增殖

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Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012Zhenjiang, ChinaRoyal Jelly is Free from Estrogens and Does Not PromoteProliferation of Human Breast Cancer MCF-7 Cells in MiceXIA Hai-Ou 1, ZHAO Yue 2, NIU Kai-jun 3, Hiroko Tani 4, KenHashimoto 4(1.The School of Nursing, Fudan University, Shanghai 200032,China; 2. The School of Nursing, Tianjin Medical University, Tianjin 300070,China; 3. Department of Epidemiology, School of Public Health, Tianjin MedicalUniversity, Tianjin, China; 4 .Institute for Bee Products and Health Science,Yamada Apiculture Center, Inc., 194 Ichiba, Kagamino 708-0393, Japan)【Background and Objective】Royal jelly (RJ), a secretion ofhoneybees (Apis mellifera), has been consumed from ancient times as a food supplement in manycountries. Its reported effectiveness includes improvement of stiff neck andsensitivity to cold in women. On the other hand, RJ has been considered to negativelyaffect breast cancer in some areas of China. In this study, we evaluated theeffect of RJ on breast cancer cells in vitro and in vivo.【Method】RJ and protease-treated RJ (pRJ) were analyzed by LC/MSfor the presence of the three estrogens estrone, estradiol, and estriol. Therate of proliferation of estrogen-responsive human breast cancer MCF-7 cells stimulatedwith estradiol in the presence of RJ and pRJ (30, 100, 300 μg/mL) wasdetermined by the AlamarBlue cell proliferation assay after 6 days of culture.In vivo, RJ and pRJ (1000 mg/kg p.o.) were administered daily to female SCIDmice transplanted with MCF-7 cells and treated with estradiol (0.5 mg/kg/di.p.) for 56 days as a model for the estrogen status in women. The effect onMCF-7 tumor growth was studied by measuring changes in the tumor volume.Tamoxifen, a medicinal substance for estrogen receptor positive breast cancer,was used as a positive control.【Result and Discussion】 No estrogen was detected in the RJor pRJ (limit of quantification : estron < 2 ppb; estradiol, estriol < 5ppb). The proliferation rate of MCF-7 cells was not promoted by the combinationof RJ or pRJ with estradiol. In mice administered estradiol for all of the testperiod, neither RJ nor pRJ significantly increased the MCF-7 tumor volume ascompared to the controls. Tamoxifen showed significant inhibition of MCF-7 cellproliferation and tumor growth. In conclusion, these results show that RJ andpRJ are free of estrogens and do not promote the proliferation of breast cancercells in vitro and in vivo. Thus, RJ is regarded as a highly safe food, andfurther study of its mechanisms of action and beneficial effects are ongoing.______________________________________________Corresponding author, E-mail：ht0807@yamada-bee.com蜂王浆ä¸�å�«æœ‰é›Œæ€§æ¿€ç´ ，并且ä¸�会促进å°�鼠体内MCF-7 乳腺癌细胞的增殖XIA Hai-Ou 1, ZHAO Yue 2, NIU Kai-jun 3, Hiroko Tani 4, KenHashimoto 4(1. å¤�旦大学护ç�†å­¦é™¢，上海 200032，中国；2.天津医科大学护ç�†å­¦é™¢，天津 300070，中国；3.中国天津医科大学æµ�行病学学院、公共å�«ç”Ÿå­¦é™¢，天津 300070，中国；4.日本蜂产å“�å’Œä¿�å�¥ç§‘学研究所，山田养蜂业中心股份有é™�å…¬å�¸，194Ichiba, Kagamino 708-0393，日本)【背景和目的】蜂王浆，一ç§�蜜蜂的分泌物（西方蜜蜂），在å�¤ä»£è®¸å¤šå›½å®¶å·²ç»�把它当å�šä¸€ç§�食物进行 食用。报告指出蜂王浆å�¯ä»¥æ”¹å–„妇女è�½æž•ä»¥å�Šå¯¹å¥³æ€§ä½“寒的体质有积æž�的效果。å�¦ä¸€æ–¹é�¢，在中国æŸ�些地区，人们认为蜂胶对乳腺癌有消æž�çš„å½±å“�。在这个研究中，我们评估了蜂王浆在人体内和人体外对乳腺癌细胞的作用。【方法】LC / MS 分æž�了RJ 和蛋白酶处ç�†RJ(pRJ)å�«æœ‰ä¸‰ä¸ªé›Œæ¿€ç´ é›Œæ¿€ç´ é…®、雌二醇和雌三醇。对于雌 性激素æ•�感的人类乳腺癌MCF-7 细胞扩散的速率å�—RJ å’ŒpRJ 里的雌二醇å�«é‡�刺激(30,100,300μg/mL) ,这个结论由AlamarBlue的细胞培养六天å�Žçš„增殖试验得出。在活体内，移æ¤�了MCF-7细胞的è�”å�ˆå…�疫缺陷鼠æ¯�天管ç�†RJå’ŒpRJ，并且用雌二醇治疗，æ ¹æ�®å¥³æ€§ä½“内雌性激素状æ€�，以56天作为一个周期。通过测é‡�è‚¿ 瘤体积的å�˜åŒ–研究MCF-7 肿瘤生长的影å“�。它莫西芬是对雌激素å�—体阳性乳腺癌的è�¯ç”¨ç‰©è´¨,被用æ�¥ä½œä¸ºä¸€ä¸ªç§¯æž�的控制。【结果和讨论内容】在RJ 或者pRJ 中未å�‘现雌性激素（é™�é‡�：雌酮< 2 ppb; 雌二醇，雌三醇< 5 ppb）。å°†rj 或者prj 和雌二醇结å�ˆæœ‰åˆ©äºŽæ��å�‡MCF-7 细胞的ç¹�殖速度。在è€�鼠身上的所有管ç�†é›ŒäºŒé†‡æµ‹è¯•æœŸé—´,无论使用RJ 或者pRJ，MCF-7 肿瘤体积对比于控制下也未显著增大。它莫西芬显著抑制细胞的增生和MCF-7肿瘤的生长。总之，这些结果表明,RJå’ŒpRJä¸�å�—雌激素影å“�，并且ä¸�促进体外和体内的乳腺癌细胞的增 æ®–。å› æ­¤,RJ是被视为æž�其安全的食物,它的作用机ç�†å’Œæœ‰ç›Šçš„å½±å“�将被æŒ�续进一步研究。