Microparticles Containing Propolis and Metronidazole: invitro Characterization, Release Study and Antimicrobial Activity AgainstPeriodontal PathogensPharm Dev Technol, 2013 Jan 28Ethylcellulose microparticles containing metronidazole andpropolis extractive solution were prepared and evaluated in vitro againstperiodontal pathogens.
Scanning electron microscopy, particle size analysis, drug entrapmentefficiency and drug release of microparticles were determined. The antimicrobialactivity of microparticles was evaluated against microorganisms of periodontalimportance (Enterococcus faecalis, Streptococcus pyogenes, Streptococcusmutans, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli). Itwas obtained particles with regular morphology, mean diameter of 1.23 µm, andentrapment efficiency for propolis and metronidazole were 91.41% and 22.23%,respectively. In vitro release studies of propolis and metronidazole frommicroparticles showed prolonged drug release and controlled by Fickiandiffusion. Both propolis and metronidazole displayed activity againstthe tested strains. Moreover, the results showed that the strains of E.faecalis, S. pyogenes and S. mutans were more susceptible to the propolis andE. faecalis to the metronidazole. It was also observed that the amount ofmetronidazole to inhibit the microorganism strains in the physical mixture withpropolis was smaller than in the metronidazole alone, suggesting potentiationeffect between propolis and metronidazole. These microparticles would be useful for developingintermediary or eventual dosage form to be administered into the periodontalpocket more easily and safely.
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