Apimedica: Bee Pollen is an Effective Natural Anti-Oxidant - 玉米蜂花粉油脂抗氧化性研究及成分分析

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Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012Zhenjiang, ChinaAntioxidant Activity and Composition Analysis of Corn BeePollen OilSUN Li-ping*, XU Xiang, HE Wei(Bee Research Institution, The Chinese Academy ofAgricultural Sciences, Beijing 100093，China)The capacity of bee pollen oil is evaluated by these threeindicators: ABTS, DPPH radical scavenging and the phosphomolybdate complex. Thecontent of carotenoid is determined by spectrophotometry, and the phospholipid compositionis quantitatively analyzed by HPLC. The results show that corn bee pollen oilshave strong scavenging ability on the ABTS, DPPH free radicals, and the IC50values are 0.26±0.01mg/mL，2.20±0.05mg/mL respectively. A strong antioxidant capacityhas also identified by the Phosphomolybdate complex method. Corn Bee Pollen, inwhich carotenoid content is 16.21 ± 0.84 mg/100g and the total phospholipidcontent is 2.98 ± 0.02g/100g, contains five phospholipid composition, namely,PI, PS, PE, PC, LPC. The proportion of carotenoids and total phospholipid ingrease is 0.21% and 38.11%. Finally, the conclusion is drawn that corn beepollen contains a variety of fat-soluble antioxidant active ingredients, cansupply hydrogen or electrons, and is an effective natural anti-oxidantresources.Key words: corn and bee pollen; antioxidant; fat-solublecomponents; content;___________________________________________*Corresponding author,E-mail：caasum@126com.cn玉米蜂花粉油脂抗氧化性研究å�Šæˆ�分分æž�孙丽è��*，å¾� å“�，何 伟（中国农业科学院蜜蜂研究所，北京 100093，中国）以ABTS、DPPH 自由基清除和磷钼络å�ˆç‰©æ³•ä¸‰ä¸ªæŒ‡æ ‡è¯„价蜂花粉油脂的抗氧化能力，通过分光光度法 测定类胡è��å�œç´ å�«é‡�，HPLC法定é‡�分æž�其中的磷脂æˆ�分。结果表明，玉米蜂花粉油脂对ABTS、DPPH 自由基有较强的清除能力，IC50 值分别为0.26±0.01mg/mL，2.20±0.05mg/mL，在磷钼络å�ˆç‰©æ³•ä¸­ä¹Ÿè¡¨çŽ°å‡ºè¾ƒ 强的抗氧化能力。玉米蜂花粉中类胡è��å�œç´ å�«é‡�为16.21±0.84mg/100g 花粉，总磷脂å�«é‡�为2.98± 0.02g/100g花粉，å�«æœ‰PI、PS、PE、PC、LPC 五ç§�磷脂æˆ�分。类胡è��å�œç´ å’Œæ€»ç£·è„‚å�«é‡�å� æ²¹è„‚的比例为0.21%å’Œ38.11%。结论：玉米蜂花粉å�«æœ‰å¤šç§�脂溶性抗氧化活性æˆ�分，有很好的供应氢或电å­�的能力，是一ç§�有效的天然抗氧化剂资æº�。关键è¯�：玉米蜂花粉；抗氧化；脂溶性æˆ�分；å�«é‡�

Apimedica: Integrated Bee Venom Therapy Helps Treat Multiple Sclerosis - 多发性硬化症之中西医结合蜂疗疗效

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Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012Zhenjiang, ChinaIntegrated Apitherapy Effects on Multiple SclerosisSiu-Wan Ip1*，CHEN Ching-yi2（1. Graduate School of Nutrition, China Medical University,Taizhong 40402, Taiwan China; 2. Asia Pacific Apitherapy Association, Zhanghua50078, Taiwan China）【Introduction】Multiple sclerosis (MS) is a commondisease in North America and Europe without effective medical treatment.Apitherapy is an effective treatment in Multiple Sclerosis patients reported inrecent years. However, most MS patients missed their golden treatment periodsbecause of allergic reactions after high dose of BVT treatments. 【Purpose】Thepurpose of this study is to investigate the effect of integrated Apitherapy(Bee venom Treatment) on Multiple Sclerosis patient. 【Method】A48-year-old female was suffering from blurred vision and weakness of limbssince 2000. Patient was admitted to Taiwan Chang-Hua Christian Hospital becauseof weakness of limbs and blurred visions. Patient developed shortness of breathand headache later. Because of lacking of improvement,she was transferred to NationalTaiwan University Hospital (NTUH) later. CT scan performed in NTUH revealed 13pathological lesions were found in her brain. The diagnosis of MultipleSclerosis was made after the CT scanning and abnormal laboratory studies ofserum and CSF. She was admitted to NTUH for medical treatments for two years. However,there was no obvious improvements after two years medical treatment in NTUH.She visited our Apitherapy clinic on September 5,2002. She was carried to ourApitherapy Clinic by her family members to receive Bee Venom Therapy (BVT)three times weekly. Blood examinations of serum Immunoglobulins (IgG, IgE) wereperformed regularly to monitor the dosages of Apitherapy. We adjust the BVT doseto maintain the serum IgG level above 1000 mg/dl during the whole BVT course.We lowered the BVT dose or stopped the BVT treatment if patient developedallergic reactions after high BVT dosing treatment. Totally 3098 bees venomwere used in four months of BVT treatments. 【Result】After received four months of BVT treatment, she startedwalking on her own on January 15,203. The blurred visions, weakness of limbsand shortness of breath were improved. 【Discussion】Multiple Sclerosis is a common disease in North Americaand Europe. There was no effective medical treatment up to present time.Apitherapy was reported to be effective in early phase of Multiple Sclerosis patients.However, most Multiple Sclerosis patients stopped BVT treatment because ofdeveloped allergic reactions after high dose of BVT. However, we shall miss thegolden treatment periods if we stopped the BVT too long. And in North Americaand Europe, family members will care about the allergic reactions after BVT. Laboratoryexaminations of serum Immulogublins regularly provide a therapy guide lines inadjusting the dosing of BVT. The combinations of Medical Nutrition Therapy(MNT) during BVT will decrease the risks of allergic reactions and enhance theeffects of BVT. High proteins diets may enhance the serum levels of IgG. Andavoiding seafood may decrease the IgE level and decrease the risk of allergicreactions.【Conclusion】Integrated Apitherapy monitoring with Immunoglobins (IgG, IgE)during the whole course of Apitherapy can increase the effect of bee venomtreatment.Key words: multiple sclerosis (MS); bee venom therapy (BVT)______________________________________________*Corresponding author,E-mail：siuwanye@gmail.com多å�‘性硬化症之中西医结å�ˆèœ‚疗疗效å�¶å…†äº‘ 1*,陈清一2(1.中国医è�¯å¤§å­¦è�¥å…»ç ”究所,å�°ä¸­40402，å�°æ¹¾ 中国；2.(中å�Ž) 亚太蜂针研究会,彰化50078, å�°æ¹¾ä¸­å›½）【å‰�言】多å�‘性硬化症(MS)为北美å�Šæ¬§æ´²å¸¸è§�之疾病,唯目å‰�ä»�有效疗法。近年求,很多医学报告蜂针 疗法对多å�‘性硬化症有疗效。但是很多M.S.病人愿接å�—高剂é‡�的蜂针疗法å�Žè¿‡æ•�,而中止B.V.T,固æ�žé”™ 失治疗M.S.的黄金时期。【目的】本研究计划为探讨中西医结å�ˆèœ‚针疗法对多å�‘性硬化症之疗效.【方法】病者庄XX,48 å²�主妇,2000 年开始出现视力å‡�退,失去四肢活动能力。病者首先ä½�å…¥å�°æ¹¾å½°åŒ– 基ç�£æ•™åŒ»é™¢。并出现呼å�¸æ€¥ä¿ƒå�Šå¤´ç—›ç­‰ç—‡çŠ¶。由于无明显疗效,病者转院到å�°åŒ—市的国立å�°æ¹¾å¤§å­¦é™„设医院 继续治疗.计算机断层扫æ��(CT scan)检查å�‘现大脑有13 个病ç�¶。血液å�ŠCS血液检查å�‡ç¬¦å�ˆå¤šå�‘性硬化症之 诊断。固此病者继续å�°å¤§åŒ»é™¢ä¹‹æ²»ç–—。但在å�°å¤§åŒ»é™¢æ²»ç–—两年å�Ž,病者并无明显的病程进步。因此病者于2002å¹´9月5日到访本蜂疗诊所。那时候,病者是由家属背ç�€æ�¥æœ¬è¯Šæ‰€å°±åŒ»。她æ¯�周回诊三次,接å�—蜂针治疗。病者定期接å�—抽血检查å…�ç–«ç�ƒè›‹ç™½（IgE、IgG）检验以调整蜂针之剂é‡�。我们在蜂针疗法全程调整IgG 指 数在1000mg/dl 以上。若病者出现过æ•�现象,我们会é™�低BVT 剂é‡�,或暂å�œBVT。病者在四个月内会全部接å�—了3098å�ªèœ‚针治疗。【结果】ç»�过四个月蜂针治疗å�Ž,病者于2003 å¹´1 月15 æ—¥å�Žèƒ½è‡ªè¡Œèµ°è·¯。视力å‡�退,呼å�¸æ€¥ä¿ƒå�Šå››è‚¢ 无力å�‡æœ‰æ˜Žæ˜¾æ”¹å–„。【讨论】多å�‘性硬化症(M.S.)为欧美常è§�之疾病,但到目å‰�为止现代医学并无有疗法。æ�®è¿‘年医学报 å‘Š,蜂针疗法对早期的M.S.有疗效。但很多病者在接å�—高剂é‡�蜂针疗法å�Ž,出现过æ•�现象,以至于蜂疗被迫 中止,因而错失治疗M.S.的黄金时期。但在欧美国家,病者家属对蜂针之过æ•�现象å��分关注。å› æ­¤,蜂针期 é—´,定期抽血,实验室检查å…�ç–«ç�ƒè›‹ç™½æŒ‡æ•°,以调整蜂针剂é‡�,å�¯ä½œä¸ºèœ‚疗的剂é‡�指导原则。å�¦å¤–,蜂针期间, 结å�ˆçŽ°ä»£åŒ»å­¦è�¥å…»ç–—法(MNT),å�¯å¢žå¼ºèœ‚针疗法之疗效å�Šé™�低过æ•�å��应。高蛋白膳食疗法å�¯æ��高血清IgG之指教。é�¿å…�摄食海产食物å�¯é™�低IgE å�Šå‡�少蜂疗过æ•�å��应。【结论】中西医结å�ˆèœ‚针疗法,以血中å…�ç–«ç�ƒè›‹ç™½(IgG,IgE)指数指导调节蜂针剂é‡�,å�¯å¢žå¼ºèœ‚疗疗效。关键è¯�:多å�‘性硬化症(M.S.)；蜂针疗法 (BVT)

Clinical Trial: The Use of Henna and Propolis on PPE

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Cyprus University of Technology
The palmar-plantar erythrodysesthesia (PPE) is the onlyclinical adverse event that commonly occurs with capecitabine and/or pegylatedliposomal doxorubicin treatment and it warrants special attention because it isthe most common dose-limiting toxicity. This study is designed to test theeffectiveness of a henna and propolis treatment protocol in the management ofcapecitabine and/or pegylated liposomal doxorubicin induced palmar-plantarerythrodysesthesia.

Apimedica: Supercritical CO2 Extraction of Propolis Produces Most Volatile Oils - 蜂胶及其不同提取物中挥发油成分的比较研究

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Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012Zhenjiang, ChinaComparative Study of Propolis and its Extract on DifferentVolatile Oil ConstituentsYang Qin, Zhou Juan, Huang Xinxin, Zhang Lin, Zhu Huifen, YuShumei, Mao Riwen(Natural Medicine Engineering Technology Research Center bySupercritical Fluid Extraction in Jiangsu province, Zhenjiang 212009,China)【Objective】To determine the amount of volatile oil in propolis glueusing the following methods: solvent extraction, propolis ethanol extractionand supercritical CO2 extraction. GC-MS was used to determine both the qualitativeand quantitative data. 【Result】The content of volatile oil in the propolis was in thefollowing order: supercritical CO2 extraction > ethanol extraction >propolis glue. The content of volatile oil in the propolis extracted bysupercritical CO2 was 23.45% higher than the propolis extracted with ethanol.The results of GC-MSindicated that propolis extracted using supercritical CO2method recorded a high content of volatile oil (54.8%) in two kinds of commonvolatile componential extracts which was superior to the propolis extractedwith ethanol. More than 1% of the composition of volatile propolis extracted bysupercritical CO2 method accounted for 64.7%. This indicated that the volatileoil composition in propolis extracted by supercritical CO2 method was betterthan the ones extracted with ethanol. Especially terpene; a kind of componentfound in the propolis extracted by supercritical CO2 was 1.5 times higher thanthe propolis extracted with ethanol. 【Conclusion】The content of propolis extracts and its volatile oil washighest in the propolis extracted by supercritical CO2. The results indicated thatthe supercritical CO2 extraction method for extracting propolis and itsvolatile oil is better than that of ethanol extraction.__________________________________________Corresponding author, E-mail: 493967536@qq.com蜂胶å�Šå…¶ä¸�å�Œæ��å�–物中挥å�‘æ²¹æˆ�分的比较研究æ�¨ç�´，周娟，黄鑫鑫，å¼ æž—，朱惠芬，éƒ�树美，毛日文（江è‹�çœ�超临界è�ƒå�–天然è�¯ç‰©å·¥ç¨‹æŠ€æœ¯ç ”究中心，镇江 212009，中国）【目的】用溶剂è�ƒå�–蜂胶原胶、蜂胶乙醇æ��å�–物å�Šèœ‚胶超临界C02æ��å�–物中的蜂胶挥å�‘æ²¹；利用GC-MS 对蜂胶挥å�‘油进行定性与定é‡�分æž�。【结果】蜂胶原胶å�Šå…¶æ��å�–物中的挥å�‘æ²¹å�«é‡�ä¾�次为蜂胶超临界C02æ��å�– 物>蜂胶乙醇æ��å�–物>蜂胶原胶。蜂胶超临界C02æ��å�–物挥å�‘æ²¹å�«é‡�较之蜂胶乙醇æ��å�–物挥å�‘æ²¹å�«é‡�高出约23.45%；通过GC-MS分æž�，两ç§�æ��å�–物共有的挥å�‘性æˆ�分中，蜂胶超临界C02æ��å�–物中å�«é‡�高的å� 54.8%，优于蜂胶乙醇æ��å�–物；在å�«é‡�＞1%的挥å�‘性æˆ�分中，蜂胶超临界CO2æ��å�–物å� 64.7%，进一步说明蜂胶超临 ç•ŒCO2æ��å�–物挥å�‘性æˆ�分优于蜂胶乙醇æ��å�–物的挥å�‘性æˆ�分。尤其是è�œçƒ¯ç±»æˆ�分，蜂胶超临界CO2æ��å�–物中 è�œçƒ¯ç±»æˆ�分å�«é‡�是蜂胶乙醇æ��å�–物中è�œçƒ¯ç±»æˆ�分å�«é‡�çš„1.5å€�。【结论】蜂胶å�Šå…¶ä¸�å�Œæ��å�–物中的挥å�‘æ²¹，以 超临界C02æ��å�–物中的å�«é‡�最高，表明利用超临界CO2æ��å�–技术æ��å�–蜂胶挥å�‘æ²¹è¦�优于乙醇æ��å�–。关键è¯�：蜂胶；超临界C02æ��å�–；乙醇æ��å�–；挥å�‘æ²¹；GC-MS

Apimedica: New ‘BeeTest’ for Detection of Viral, Bacterial Diseases of Honey Bees – “蜜蜂测试”—免疫色谱法快速检测蜜蜂病毒和细菌疾病

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Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012Zhenjiang, ChinaImmunochromatographic Rapid Test “BeeTest” for Detection of Viraland Bacterial Diseases of Honey Bees Apis melliferaLeonid Kelin（“BeeTest” Ltd, Russian Federation,Novosibirsk, Russia）Russian company "Beetest" presents a product whichis unique in the world - immunochromatographic rapid test "Beetest."As our company produces a preparation “Endoglukin” for prevention and treatmentof viral diseases of honey bees and stimulation of the bee colony development,we decided to develop a diagnostic test as a prior stage for diseases treatmentin order to know exactly what kind of disease should be treated. After severalyears of researches we finally managed to find specific antigens for eachdisease that ensures the high sensitivity and delicacy of our test.With the test “Beetest” you can simultaneously check yourhives for the presence of the major bacterial and viral diseases of bees. Thetest is intended for use in the field, does not require highly trainedpersonnel and can be used directly next to the hive.Principle of the methodImmunochromatographic rapid test “BeeTest” is asandwich-type lateral flow assay for simultaneous and differential detection oftwo viral and two bacterial honey bee diseases:· Acute bee paralysis virus;· Sacbrood virus;· American foulbrood (Paenibacillus larvae);· European foulbrood (Melissococcus plutonius)“BeeTest” was developed for rapid, quality and one-stepdetection of the viral and bacterial proteins in honey bee larvae byLateral-flow immunochromatographic technology. The principal scheme of the testis on the picture.
The nitrocellulose membrane is coated with specificpolyclonal antibodies to proteins of Acute bee paralysis virus or Sacbroodvirus or American foulbrood (Paenibacillus larvae) or European foulbrood (Melissococcusplutonius) and specific proteins of these infectious agents.During the first step disrupt the honey bee larvae in thebottle with Elution buffer and glass balls by vigorous shaking. On the secondstep drip a couple of drops into the sample window. In this moment specificviral or bacterial proteins (if they are present in the sample) forms thecomplex with the conjugates of colloidal gold with poly- or monoclonalantibodies.Further these complex bind with the specific antibodiescoated on the membrane thus forming the red line (test line). Conjugates whichwere not involved in forming the complex with bacterial or viral proteins bindswith the specific proteins on the membrane (Picture 2) thus forming another redline (control line). The intensity of red color lines should be assessedvisually.
Reagents and materials:1) 4 plastic cassettes (one cassette for one disease);2) Plastic bottle with a glass balls and Elution buffer;3) Single-use pipette;4) Single-use spatula.Assay procedure:All reagents are ready-to-use and doesn’t need in anypreparations.1) Extract a larva showing suspicious symptoms with thespatula.2) Unscrew lid from the Bottle with Elution buffer. Use thespatula to place sample in the bottle. Replace the lid and shake vigorously forabout 20 seconds.Test line Control line3) Unscrew lid of Bottle and pipe the sample by suppliedpipette. One honey bee larvae should be used with all types of cassettes.4) Hold the Test cassette horizontally and gently squeezetwo drops onto the sample well of the each cassette.5) Keep it horizontal until extract is absorbed (c 30seconds) and a red dye appears in the viewing window.6) Wait until the control line appears (labeled C) and readthe result (10-15 minutes).Reading the results3 Positive result - two lines show up (Test and Control) -indicates that the target pathogen is present in the test sample.4 Negative result - Control line shows up only, no Test line- indicates that the target pathogen has not been detected in the test sample.5 Indefinite result - one Test line without Control line orno one lines indicates that test doesn’t work.Analytical sensitivity of the test kitAnalytical sensitivity is 10-50 ng/ml with using ofrecombinants proteins of Acute bee paralysis virus, Sacbrood virus, American foulbrood (Paenibacillus larvae),European foulbrood (Melissococcus plutonius). Shelf life, storage and transportation conditionsShelf life of the “BeeTest” is 12 month from the date ofmanufacture. Store at 17-27 °C in the dry dark place.______________________________________________*Corresponding author, E-mail: beetest.org@gmail.com“蜜蜂测试”—å…�疫色谱法快速检测蜜蜂病毒和细è�Œç–¾ç—…Leonid Kelin （ä¿„ç½—æ–¯）（“BeeTest” Ltd, Russian Federation,Novosibirsk, Russia）俄罗斯公å�¸çš„“蜜蜂测试”是世界上一ç§�先进独特的快速å…�疫色谱法。本公å�¸ç”¨æ­¤æ³•æŽ§åˆ¶å’Œæ²»ç–—蜜蜂疾病和刺激蜜蜂ç§�群的å�‘展，以达到更好的制备Endoglukin，å…ˆå‰�我们决定研å�‘一ç§�诊断测试法，为了在蜜蜂疾病治疗å‰�准确定ä½�疾病的ç§�ç±»。ç»�过几年的研究，我们最终找到æ¯�一ç§�疾病的特异性抗原，è¿™ä¿�è¯�测试的高效ç�µæ•�。有了蜜蜂测试，ä½ å�¯ä»¥å�Œæ—¶æ£€æŸ¥ä½ èœ‚群主è¦�疾病细è�Œå’Œç—…毒。本测试ä¸�需è¦�专业技术人员，å�¯ç›´æŽ¥åœ¨èœ‚ç®±æ—�边进行检测。方法原ç�†èœœèœ‚测试，快速å…�疫色谱法，是一ç§�夹心型横æµ�化测试法，å�¯å�Œæ—¶å¯¹ä¸¤ç§�蜜蜂疾病病毒和细è�Œè¿›è¡Œç¤º 差检测。1. 蜜蜂急性麻痹肠é�“病毒2. 幼蜂皱è�Žç—…病毒3. 美洲蜜蜂幼虫è…�臭病4. 欧洲蜜蜂幼虫è…�臭病通过横æµ�å…�疫色谱技术，蜜蜂测试å�¯å¿«é€Ÿ，高效，一步检测出蜜蜂幼虫中病毒和细è�Œè›‹ç™½，它主è¦�çš„ 测试路线如图1 所示：ç¡�酸纤维素膜上涂有蜜蜂急性麻痹肠é�“病毒，幼蜂皱è�Žç—…病毒，美洲蜜蜂幼虫è…�臭病毒和欧洲蜜蜂幼虫è…�臭病毒的多克隆抗体以å�Šè¿™äº›ä¼ æŸ“æº�的特殊蛋白。第一步将蜜蜂幼虫放入带有洗脱缓冲液和玻璃ç�ƒçš„瓶中，剧烈振è�¡。第二步滴几滴试剂在采样窗å�£ä¸­，此时特殊病毒和细è�Œè›‹ç™½（如果样å“�中存在）将与å�•å…‹éš†æŠ—体和胶体金的轭å�ˆç‰©å½¢æˆ�å¤�å�ˆç‰©。图二测试线和控制线中轭å�ˆç‰©å’ŒæŠ—原的键å�ˆ（Au–胶体金和抗体的轭å�ˆç‰©, Ag – 抗原) 之å�Žè¿™äº›å¤�å�ˆç‰©å’Œè–„膜上的特殊抗体紧紧地结å�ˆåœ¨ä¸€èµ·，å½¢æˆ�红线（检测线），那些没有没有和薄膜上 的特殊蛋白结å�ˆçš„å¤�å�ˆç‰©（如图二）å½¢æˆ�å�¦ä¸€æ�¡çº¢çº¿（控制线），红色线的强度å�¯ä»¥ç”¨çœ¼è§‚测出æ�¥。试剂和æ��æ–™1. 4 个塑料储片夹（一个对应一ç§�疾病）2.带有玻璃ç�ƒå’Œæ´—脱缓冲液的塑料瓶3.一次性试管4.一次性è�¯åˆ€å®žéªŒæ­¥éª¤æ‰€æœ‰è¯•å‰‚都è¦�æ��å‰�准备好。1. 用è�¯åˆ€å�–一å�ªæœ‰å�¯ç–‘症状的幼虫2. 旋开带有洗脱缓冲液的塑料瓶，用è�¯åˆ€æŠŠå¹¼è™«æ”¾å…¥å¡‘料瓶中。盖上瓶盖，剧烈振è�¡20 秒。3. 旋开瓶盖，用å�¸é‡�管å�¸å�–洗脱缓冲液。æ¯�个幼虫的缓冲液液都è¦�æ»´å…¥4 个塑料储片夹。4. 水平拿ä½�测试储片夹，缓慢滴两滴缓冲液在样å“�室。5. ä¿�æŒ�æ°´å¹³，使æ��å�–物被å�¸æ”¶（30 秒），观察窗中出现红线。6. 等到控制线出现（标记为C）然å�Žè¯»å‡ºç»“æžœ（10-15 分钟）结果1、阳性结果——出现两æ�¡çº¢çº¿（检测线和控制线）—表明目标病原体出现在测试样å“�中。2、阴性结果——å�ªå‡ºçŽ°æŽ§åˆ¶çº¿—表明目标病原体ä¸�存在于测试样å“�中。3、无效结果——有测检测线没有控制线或者两æ�¡éƒ½æ²¡æœ‰è¡¨æ˜Žæµ‹è¯•æ— æ•ˆè¯•å‰‚盒的分æž�ç�µæ•�度用10-50ng/mg蜜蜂急性麻痹肠é�“病毒，幼蜂皱è�Žç—…病毒，美洲蜜蜂幼虫è…�臭病毒和欧洲蜜蜂幼虫è…�臭病毒的é‡�组蛋白质æ�¥åˆ†æž�ç�µæ•�度。ä¿�质期，储存和è¿�输æ�¡ä»¶ ä¿�质期12 个月，储存æ�¡ä»¶ä¸º17-27℃，黑暗干燥处。

Apimedica: Bee Venom Dose Should Be Adjusted for Each Person, Illness - 蜂针疗法用蜂量的思考

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Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012Zhenjiang, ChinaUnderstanding of Bee Dose in Bee Needle TherapyWANLAI Si-qi, LI Wan-yao*, CAO Xi-jun(Acupucture and Massage College, Guangzhou University ofTCM, Guangzhou 510405, China)Bee needle therapy was widely welcomed by patients, whilethere was no standards of bee dose in clinical. For the same disease, some onlyuse a few bees to obtain good results, some use more than hundreds bees. There aregreat risks and dangers. This article explores this issue. The dose of bee should be adjusted according to different people,time and illness. A new patient with a small dose is better. Bee Sting quantitygenerally starts from 1-2, the allergy period maintain 1-3 is appropriate. Thedose of patients who had bee needle therapy for a long time may be appropriateto increase, generally maintained at about 10. Severe cases may whereappropriate, increase to 30 to 50. Physically weak, sick light, re-treatmentshould be a small amount of bee needle treatment. Physically strong, theseriously ill who may be appropriate to increase the bee amount. For patientswith light disease 1 time a week or two week, they often recover after string1-3 times. For patients with general acute or severe diseases, once a day,continuing for 2-3 courses or more of treatment. For prevention of recurrence,consider once a month. Small bee dose can’t treat the disease, excessive leadto serious consequences. Strictly control the amount of bee, in order toalleviate the suffering of patients with the most appropriate amount.Corresponding author, E-mail：liwanyao@yahoo.com.cn蜂针疗法用蜂é‡�çš„æ€�考万赖æ€�ç�ª,æ�Žä¸‡ç‘¶*, 曹喜俊（广州中医è�¯å¤§å­¦，广州 510405,中国）蜂针疗法在å�—到患者的广泛欢迎的å�Œæ—¶，临床上的用蜂é‡�尚无规范，有的仅用数å�ªèœ‚治疗å�–得良好的 效果，有的用上百å�ªæ²»ç–—å�Œæ ·çš„疾患，存在æž�大的风险和å�±é™©。本文针对于此探讨了蜂针疗法用é‡�，认为：蜂针é‡�应因人、å› æ—¶、因病施治。新患者少é‡�为佳，1～2 å�ªå§‹，过æ•�期内维æŒ�1～3 å�ªä¸ºå®œ；久蜂å�¯é€‚当加 é‡�，一般维æŒ�在10 å�ªå·¦å�³，严é‡�者å�¯é…Œæƒ…加大至30～50 å�ª。体虚、ç—…è½»、å¤�针者宜少é‡�蜂针治疗，体实、ç—…é‡�者å�¯é€‚当增加蜂é‡�。病情轻浅者å�¯ä»¥æ¯�周或隔周1 次，1～3 次å�¯æ„ˆ；急性病、严é‡�病者需æ¯�æ—¥1 次，æŒ�ç»­2～3 个疗程，或更久；预防å¤�å�‘å�¯1 月1 次。å°�蜂é‡�难以愈病，过é‡�易导致严é‡�å�Žæžœ，è¦�严格把æ�¡å¥½èœ‚针的用é‡�，以期用最å�ˆé€‚çš„é‡�缓解患者的痛苦。