Apimedica: Bee Pollen is an Effective Natural Anti-Oxidant - 玉米蜂花粉油脂抗氧化性研究及成分分析

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Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012Zhenjiang, ChinaAntioxidant Activity and Composition Analysis of Corn BeePollen OilSUN Li-ping*, XU Xiang, HE Wei(Bee Research Institution, The Chinese Academy ofAgricultural Sciences, Beijing 100093，China)The capacity of bee pollen oil is evaluated by these threeindicators: ABTS, DPPH radical scavenging and the phosphomolybdate complex. Thecontent of carotenoid is determined by spectrophotometry, and the phospholipid compositionis quantitatively analyzed by HPLC. The results show that corn bee pollen oilshave strong scavenging ability on the ABTS, DPPH free radicals, and the IC50values are 0.26±0.01mg/mL，2.20±0.05mg/mL respectively. A strong antioxidant capacityhas also identified by the Phosphomolybdate complex method. Corn Bee Pollen, inwhich carotenoid content is 16.21 ± 0.84 mg/100g and the total phospholipidcontent is 2.98 ± 0.02g/100g, contains five phospholipid composition, namely,PI, PS, PE, PC, LPC. The proportion of carotenoids and total phospholipid ingrease is 0.21% and 38.11%. Finally, the conclusion is drawn that corn beepollen contains a variety of fat-soluble antioxidant active ingredients, cansupply hydrogen or electrons, and is an effective natural anti-oxidantresources.Key words: corn and bee pollen; antioxidant; fat-solublecomponents; content;___________________________________________*Corresponding author,E-mail：caasum@126com.cn玉米蜂花粉油脂抗氧化性研究å�Šæˆ�分分æž�孙丽è��*，å¾� å“�，何 伟（中国农业科学院蜜蜂研究所，北京 100093，中国）以ABTS、DPPH 自由基清除和磷钼络å�ˆç‰©æ³•ä¸‰ä¸ªæŒ‡æ ‡è¯„价蜂花粉油脂的抗氧化能力，通过分光光度法 测定类胡è��å�œç´ å�«é‡�，HPLC法定é‡�分æž�其中的磷脂æˆ�分。结果表明，玉米蜂花粉油脂对ABTS、DPPH 自由基有较强的清除能力，IC50 值分别为0.26±0.01mg/mL，2.20±0.05mg/mL，在磷钼络å�ˆç‰©æ³•ä¸­ä¹Ÿè¡¨çŽ°å‡ºè¾ƒ 强的抗氧化能力。玉米蜂花粉中类胡è��å�œç´ å�«é‡�为16.21±0.84mg/100g 花粉，总磷脂å�«é‡�为2.98± 0.02g/100g花粉，å�«æœ‰PI、PS、PE、PC、LPC 五ç§�磷脂æˆ�分。类胡è��å�œç´ å’Œæ€»ç£·è„‚å�«é‡�å� æ²¹è„‚的比例为0.21%å’Œ38.11%。结论：玉米蜂花粉å�«æœ‰å¤šç§�脂溶性抗氧化活性æˆ�分，有很好的供应氢或电å­�的能力，是一ç§�有效的天然抗氧化剂资æº�。关键è¯�：玉米蜂花粉；抗氧化；脂溶性æˆ�分；å�«é‡�