Apimedica: Supercritical CO2 Extraction of Propolis Produces Most Volatile Oils - 蜂胶及其不同提取物中挥发油成分的比较研究

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Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012Zhenjiang, ChinaComparative Study of Propolis and its Extract on DifferentVolatile Oil ConstituentsYang Qin, Zhou Juan, Huang Xinxin, Zhang Lin, Zhu Huifen, YuShumei, Mao Riwen(Natural Medicine Engineering Technology Research Center bySupercritical Fluid Extraction in Jiangsu province, Zhenjiang 212009,China)【Objective】To determine the amount of volatile oil in propolis glueusing the following methods: solvent extraction, propolis ethanol extractionand supercritical CO2 extraction. GC-MS was used to determine both the qualitativeand quantitative data. 【Result】The content of volatile oil in the propolis was in thefollowing order: supercritical CO2 extraction > ethanol extraction >propolis glue. The content of volatile oil in the propolis extracted bysupercritical CO2 was 23.45% higher than the propolis extracted with ethanol.The results of GC-MSindicated that propolis extracted using supercritical CO2method recorded a high content of volatile oil (54.8%) in two kinds of commonvolatile componential extracts which was superior to the propolis extractedwith ethanol. More than 1% of the composition of volatile propolis extracted bysupercritical CO2 method accounted for 64.7%. This indicated that the volatileoil composition in propolis extracted by supercritical CO2 method was betterthan the ones extracted with ethanol. Especially terpene; a kind of componentfound in the propolis extracted by supercritical CO2 was 1.5 times higher thanthe propolis extracted with ethanol. 【Conclusion】The content of propolis extracts and its volatile oil washighest in the propolis extracted by supercritical CO2. The results indicated thatthe supercritical CO2 extraction method for extracting propolis and itsvolatile oil is better than that of ethanol extraction.__________________________________________Corresponding author, E-mail: 493967536@qq.com蜂胶å�Šå…¶ä¸�å�Œæ��å�–物中挥å�‘æ²¹æˆ�分的比较研究æ�¨ç�´，周娟，黄鑫鑫，å¼ æž—，朱惠芬，éƒ�树美，毛日文（江è‹�çœ�超临界è�ƒå�–天然è�¯ç‰©å·¥ç¨‹æŠ€æœ¯ç ”究中心，镇江 212009，中国）【目的】用溶剂è�ƒå�–蜂胶原胶、蜂胶乙醇æ��å�–物å�Šèœ‚胶超临界C02æ��å�–物中的蜂胶挥å�‘æ²¹；利用GC-MS 对蜂胶挥å�‘油进行定性与定é‡�分æž�。【结果】蜂胶原胶å�Šå…¶æ��å�–物中的挥å�‘æ²¹å�«é‡�ä¾�次为蜂胶超临界C02æ��å�– 物>蜂胶乙醇æ��å�–物>蜂胶原胶。蜂胶超临界C02æ��å�–物挥å�‘æ²¹å�«é‡�较之蜂胶乙醇æ��å�–物挥å�‘æ²¹å�«é‡�高出约23.45%；通过GC-MS分æž�，两ç§�æ��å�–物共有的挥å�‘性æˆ�分中，蜂胶超临界C02æ��å�–物中å�«é‡�高的å� 54.8%，优于蜂胶乙醇æ��å�–物；在å�«é‡�＞1%的挥å�‘性æˆ�分中，蜂胶超临界CO2æ��å�–物å� 64.7%，进一步说明蜂胶超临 ç•ŒCO2æ��å�–物挥å�‘性æˆ�分优于蜂胶乙醇æ��å�–物的挥å�‘性æˆ�分。尤其是è�œçƒ¯ç±»æˆ�分，蜂胶超临界CO2æ��å�–物中 è�œçƒ¯ç±»æˆ�分å�«é‡�是蜂胶乙醇æ��å�–物中è�œçƒ¯ç±»æˆ�分å�«é‡�çš„1.5å€�。【结论】蜂胶å�Šå…¶ä¸�å�Œæ��å�–物中的挥å�‘æ²¹，以 超临界C02æ��å�–物中的å�«é‡�最高，表明利用超临界CO2æ��å�–技术æ��å�–蜂胶挥å�‘æ²¹è¦�优于乙醇æ��å�–。关键è¯�：蜂胶；超临界C02æ��å�–；乙醇æ��å�–；挥å�‘æ²¹；GC-MS