Food Chem, 2013 Feb 15;136(3-4):1577-83 Pyrrolizidine alkaloids (PAs) are knownsecondary plant metabolites which can cause hepatotoxicity in both humans andlivestock. PAs can be consumed through the use of plants for food, medicinalpurposes and as contaminants of agricultural crops and food. PA contaminatedgrain has posed the largest health risk, although any PA contamination in ourfood chain should be recognised as a potential health threat. For this purpose,retail honeys were tested by LC-MS/MS. The method allows for specificidentification of toxic retronecine and otonecine-type PAs by comparison toreference compounds via a spectral library. In total, 50 honey samples werematched to the reference spectra within a set of tolerance parameters. Accuratedata analysis and quick detection of positive samples was possible. Positivesamples contained an average PA concentration of 1260μgkg(-1) of honey. Goodlinear calibrations were obtained (R(2)>0.991). LOD and LOQ ranged from0.0134 to 0.0305 and 0.0446 to 0.1018μgmL(-1), respectively.
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