Apimedica: Antioxidant Activity of Royal Jelly Protease Hydrolyzate in vitro - 蜂王浆蛋白酶解物体外抗氧化活性的研究

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Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012Zhenjiang, China LI Yi-wu, YANG Wen-chao, WU Zhen-hong, MIAO Xiao-qing*(Bee Science College of Fujian Agriculture and ForestryUniversity, Honey products processing and application engineering researchcenter of ministry of education, Fuzhou 350002, China)The health care function of royal jelly is mainly reflectedon its antioxidant capacity, and its functional active ingredients are, in alarge extent, related with its unique protein. Royal jelly protein is absorbedthrough the enzymolysis of pepsin, trypsin, and chymotrypsin into peptides. Bydetermining the radical scavenging ability, hydroxyl radical scavengingability, reducing ability and anti-lipid oxidation ability of the royal jellyprotein hydrolyzate on DPPH, the best water resolutions for DPPH’s free radicalscavenging ability, hydroxyl radical scavenging ability, reducing power abilityand anti-lipid peroxidation ability were obtained. And the best water resolutionswere 4.18%, 4.50%, 4.18%, and 4.50% respectively for the abilities mentionedabove.Key words: royal jelly; protease hydrolyzate; antioxidant__________________________________________*Corresponding author, E-mail：fjbees@126.com蜂王浆蛋白酶解物体外抗氧化活性的研究æ�Žç›Šæ­¦，æ�¨æ–‡è¶…，å�´ç��红，缪晓é�’*（ç¦�建农林大学蜂疗研究所，教育部蜂产å“�加工与应用工程中心，ç¦�å·ž 350002，中国）蜂王浆的ä¿�å�¥åŠŸèƒ½ä¸»è¦�体现在其抗氧化能力上，其功能活性æˆ�分å�ˆå¾ˆå¤§ç¨‹åº¦ä¸Šä¸Žå…¶æ‰€ç‰¹æœ‰çš„蛋白质有 å…³。王浆蛋白通过人体的胃蛋白酶、胰蛋白酶、胰å‡�乳蛋白酶酶解æˆ�多肽在人体å�¸æ”¶åˆ©ç”¨。由通过王浆蛋白酶解物对DPPH 自由基清除能力、羟自由基清除能力、还原力和抗脂质过氧化能力的测定，得出DPPH自由基清除能力、羟自由基清除能力、还原力和抗脂质过氧化能力的最佳水解度分别为4.18%、4.50%、4.18%、4.50%。关键è¯�：蜂王浆；酶解物；抗氧化