Consumption of Bee Pollen Affects Rat Ovarian FunctionsJ Anim Physiol Anim Nutr (Berl), 2012 Nov 9http://onlinelibrary.wiley.com/doi/10.1111/jpn.12013/abstractThe aim of this study was to examine possible effects of beepollen added to the feed mixture (FM) on rat ovarian functions (secretionactivity and apoptosis). We evaluated the bee pollen effect on the release ofinsulin-like growth factor I (IGF-I) and steroid hormones (progesterone andestradiol), as well as on the expression of markers of apoptosis (Bcl-2, Baxand caspase-3) in rat ovarian fragments. Female rats (n = 15) were fed during90 days by FM without or with rape seed bee pollen in dose either 3 kg/1000 kgFM or 5 kg/1000 kg FM. Fragments of ovaries isolated from rats of each group(totally 72 pieces) were incubated for 24 h. Hormonal secretion into theculture medium was detected by RIA. The markers of apoptosis were evaluated byWestern blotting. It was observed that IGF-I release by rat ovarian fragmentswas significantly decreased; on the other hand, progesterone andestradiol secretion was increased after bee pollen treatment at dose5 kg/1000 kg FM but not at 3 kg/1000 FM. Accumulation of Bcl-2 was increased bybee pollen added at 3 kg/1000 kg FM, but not at higher dose. Accumulation ofBax was increased in ovaries of rats fed by bee pollen at doses either 3 or5 kg/1000 kg FM, whilst accumulation of caspase-3 increased after feeding withbee pollen at dose 5 kg/1000 kg FM, but not at 3 kg/1000 kg FM. Our resultscontribute to new insights regarding the effect of bee pollen on both secretionactivity (release of growth factor IGF-I and steroid hormones progesterone andestradiol) and apoptosis (anti- and pro-apoptotic markers Bcl-2, Bax andcaspase-3). Bee pollen is shown to be a potent regulator of rat ovarianfunctions.
17 Kasım 2012 Cumartesi
Bee Pollen a Potent Regulator of Ovarian Functions
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Consumption of Bee Pollen Affects Rat Ovarian FunctionsJ Anim Physiol Anim Nutr (Berl), 2012 Nov 9http://onlinelibrary.wiley.com/doi/10.1111/jpn.12013/abstractThe aim of this study was to examine possible effects of beepollen added to the feed mixture (FM) on rat ovarian functions (secretionactivity and apoptosis). We evaluated the bee pollen effect on the release ofinsulin-like growth factor I (IGF-I) and steroid hormones (progesterone andestradiol), as well as on the expression of markers of apoptosis (Bcl-2, Baxand caspase-3) in rat ovarian fragments. Female rats (n = 15) were fed during90 days by FM without or with rape seed bee pollen in dose either 3 kg/1000 kgFM or 5 kg/1000 kg FM. Fragments of ovaries isolated from rats of each group(totally 72 pieces) were incubated for 24 h. Hormonal secretion into theculture medium was detected by RIA. The markers of apoptosis were evaluated byWestern blotting. It was observed that IGF-I release by rat ovarian fragmentswas significantly decreased; on the other hand, progesterone andestradiol secretion was increased after bee pollen treatment at dose5 kg/1000 kg FM but not at 3 kg/1000 FM. Accumulation of Bcl-2 was increased bybee pollen added at 3 kg/1000 kg FM, but not at higher dose. Accumulation ofBax was increased in ovaries of rats fed by bee pollen at doses either 3 or5 kg/1000 kg FM, whilst accumulation of caspase-3 increased after feeding withbee pollen at dose 5 kg/1000 kg FM, but not at 3 kg/1000 kg FM. Our resultscontribute to new insights regarding the effect of bee pollen on both secretionactivity (release of growth factor IGF-I and steroid hormones progesterone andestradiol) and apoptosis (anti- and pro-apoptotic markers Bcl-2, Bax andcaspase-3). Bee pollen is shown to be a potent regulator of rat ovarianfunctions.
Consumption of Bee Pollen Affects Rat Ovarian FunctionsJ Anim Physiol Anim Nutr (Berl), 2012 Nov 9http://onlinelibrary.wiley.com/doi/10.1111/jpn.12013/abstractThe aim of this study was to examine possible effects of beepollen added to the feed mixture (FM) on rat ovarian functions (secretionactivity and apoptosis). We evaluated the bee pollen effect on the release ofinsulin-like growth factor I (IGF-I) and steroid hormones (progesterone andestradiol), as well as on the expression of markers of apoptosis (Bcl-2, Baxand caspase-3) in rat ovarian fragments. Female rats (n = 15) were fed during90 days by FM without or with rape seed bee pollen in dose either 3 kg/1000 kgFM or 5 kg/1000 kg FM. Fragments of ovaries isolated from rats of each group(totally 72 pieces) were incubated for 24 h. Hormonal secretion into theculture medium was detected by RIA. The markers of apoptosis were evaluated byWestern blotting. It was observed that IGF-I release by rat ovarian fragmentswas significantly decreased; on the other hand, progesterone andestradiol secretion was increased after bee pollen treatment at dose5 kg/1000 kg FM but not at 3 kg/1000 FM. Accumulation of Bcl-2 was increased bybee pollen added at 3 kg/1000 kg FM, but not at higher dose. Accumulation ofBax was increased in ovaries of rats fed by bee pollen at doses either 3 or5 kg/1000 kg FM, whilst accumulation of caspase-3 increased after feeding withbee pollen at dose 5 kg/1000 kg FM, but not at 3 kg/1000 kg FM. Our resultscontribute to new insights regarding the effect of bee pollen on both secretionactivity (release of growth factor IGF-I and steroid hormones progesterone andestradiol) and apoptosis (anti- and pro-apoptotic markers Bcl-2, Bax andcaspase-3). Bee pollen is shown to be a potent regulator of rat ovarianfunctions.
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