Synergistic Effects of Honey and Propolis Toward DrugMulti-Resistant Staphylococcus Aureus, Escherichia Coli and Candida AlbicansIsolates in Single and Polymicrobial CulturesInt J Med Sci, 2012;9(9):793-800Background: Propolis and honey are natural bee products withwide range of biological and medicinal properties. The study investigatedantimicrobial activity of ethyl alcohol extraction of propolis collected fromSaudi Arabia (EEPS) and from Egypt (EEPE), and their synergistic effect whenused with honey. Single and polymicrobial cultures of antibiotic resistanthuman pathogens were tested.Material and methods; Staphylococcus aureus (S. aureus),),Escherichia coli (E. coli) and Candida albicans (C.albicans) were cultured in10-100% (v/v) honey diluted in broth, or 0.08-1.0% (weight/volume) EEPS andEEPE diluted in broth. Four types of polymicrobial cultures were prepared byculturing the isolates with each other in broth (control) and broth containingvarious concentrations of honey or propolis. Microbial growth was assessed onsolid plate media after 24 h incubation.Results; EEPS and EEPE inhibited antibiotic resistantE.coli, and S.aureus, and C.albicans in single and polymicrobial cultures.S.aureus became more susceptible when it was cultured with E.coli or C.albicansor when all cultured together. C.albicans became more susceptible when it wascultured with S.aureus or with E.coli and S. aureus together. The presence ofethyl alcohol or honey potentiated antimicrobial effect of propolis towardentire microbes tested in single or polymicrobial cultures. EEPS had lower MICtoward E.coli and C.albicans than EEPE. When propolis was mixed with honey,EEPS showed lower MIC than EEPE. In addition, honey showed lower MIC towardentire microbes when mixed with EEPS than when it was mixed with EEPE.Conclusion; 1) propolis prevents the growth of themicroorganisms in single and mixed microbial cultures, and has synergisticeffect when used with honey or ethyl alcohol, 2) the antimicrobial property ofpropolis varies with geographical origin, and 3) this study will pave the wayto isolate active ingredients from honey and propolis to be further testedindividually or in combination against human resistant infections
17 Kasım 2012 Cumartesi
Propolis has Synergistic Antimicrobial Effect When Used with Honey
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Synergistic Effects of Honey and Propolis Toward DrugMulti-Resistant Staphylococcus Aureus, Escherichia Coli and Candida AlbicansIsolates in Single and Polymicrobial CulturesInt J Med Sci, 2012;9(9):793-800Background: Propolis and honey are natural bee products withwide range of biological and medicinal properties. The study investigatedantimicrobial activity of ethyl alcohol extraction of propolis collected fromSaudi Arabia (EEPS) and from Egypt (EEPE), and their synergistic effect whenused with honey. Single and polymicrobial cultures of antibiotic resistanthuman pathogens were tested.Material and methods; Staphylococcus aureus (S. aureus),),Escherichia coli (E. coli) and Candida albicans (C.albicans) were cultured in10-100% (v/v) honey diluted in broth, or 0.08-1.0% (weight/volume) EEPS andEEPE diluted in broth. Four types of polymicrobial cultures were prepared byculturing the isolates with each other in broth (control) and broth containingvarious concentrations of honey or propolis. Microbial growth was assessed onsolid plate media after 24 h incubation.Results; EEPS and EEPE inhibited antibiotic resistantE.coli, and S.aureus, and C.albicans in single and polymicrobial cultures.S.aureus became more susceptible when it was cultured with E.coli or C.albicansor when all cultured together. C.albicans became more susceptible when it wascultured with S.aureus or with E.coli and S. aureus together. The presence ofethyl alcohol or honey potentiated antimicrobial effect of propolis towardentire microbes tested in single or polymicrobial cultures. EEPS had lower MICtoward E.coli and C.albicans than EEPE. When propolis was mixed with honey,EEPS showed lower MIC than EEPE. In addition, honey showed lower MIC towardentire microbes when mixed with EEPS than when it was mixed with EEPE.Conclusion; 1) propolis prevents the growth of themicroorganisms in single and mixed microbial cultures, and has synergisticeffect when used with honey or ethyl alcohol, 2) the antimicrobial property ofpropolis varies with geographical origin, and 3) this study will pave the wayto isolate active ingredients from honey and propolis to be further testedindividually or in combination against human resistant infections
Synergistic Effects of Honey and Propolis Toward DrugMulti-Resistant Staphylococcus Aureus, Escherichia Coli and Candida AlbicansIsolates in Single and Polymicrobial CulturesInt J Med Sci, 2012;9(9):793-800Background: Propolis and honey are natural bee products withwide range of biological and medicinal properties. The study investigatedantimicrobial activity of ethyl alcohol extraction of propolis collected fromSaudi Arabia (EEPS) and from Egypt (EEPE), and their synergistic effect whenused with honey. Single and polymicrobial cultures of antibiotic resistanthuman pathogens were tested.Material and methods; Staphylococcus aureus (S. aureus),),Escherichia coli (E. coli) and Candida albicans (C.albicans) were cultured in10-100% (v/v) honey diluted in broth, or 0.08-1.0% (weight/volume) EEPS andEEPE diluted in broth. Four types of polymicrobial cultures were prepared byculturing the isolates with each other in broth (control) and broth containingvarious concentrations of honey or propolis. Microbial growth was assessed onsolid plate media after 24 h incubation.Results; EEPS and EEPE inhibited antibiotic resistantE.coli, and S.aureus, and C.albicans in single and polymicrobial cultures.S.aureus became more susceptible when it was cultured with E.coli or C.albicansor when all cultured together. C.albicans became more susceptible when it wascultured with S.aureus or with E.coli and S. aureus together. The presence ofethyl alcohol or honey potentiated antimicrobial effect of propolis towardentire microbes tested in single or polymicrobial cultures. EEPS had lower MICtoward E.coli and C.albicans than EEPE. When propolis was mixed with honey,EEPS showed lower MIC than EEPE. In addition, honey showed lower MIC towardentire microbes when mixed with EEPS than when it was mixed with EEPE.Conclusion; 1) propolis prevents the growth of themicroorganisms in single and mixed microbial cultures, and has synergisticeffect when used with honey or ethyl alcohol, 2) the antimicrobial property ofpropolis varies with geographical origin, and 3) this study will pave the wayto isolate active ingredients from honey and propolis to be further testedindividually or in combination against human resistant infections
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